Proceed to sample analysis by western immunoblotting or kinase activity section D. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Adjust pH to 8. Discard supernatant in appropriate waste container. Biotinylated Protein Ladder Detection Pack: Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies:
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Proceed with Immunostaining Section C.
Recommended Anti-Rabbit secondary dp Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Electrotransfer to nitrocellulose membrane Transfer supernatant containing phosphorylated substrate to another tube. Scrape cells off the plate and transfer to microcentrifuge tubes.
Detection of Proteins Directions for Use: To Purchase S View sizes. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Wash three times for 5 min each with 15 ml of TBST.
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Biotinylated Protein Ladder Detection Pack: Incubate sections in three washes of xylene for 5 min each. Staining Wash sections in dH 2 O three times for 5 min each.
Repeat in xylene, incubating sections two times for 10 sec each. While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer.
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Application Dilutions Western Blotting 1: An unknown error occured To fix the EV problem if you’ve got one 988, head to the following MS document: To start the conversation again, simply ask a new question.
Dec 16, 7: Allow cells to fix for 15 min at room temperature.
Proceed with Immunostaining Section C. It contains a LEM domain and binds to several nuclear proteins, such as BAF barrier-to-autointegration factor and A- and B-type lamins, which are important in nuclear functions Wash sections three times with wash buffer for 5 min each. Would you like to visit your country specific website?
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Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, 8908 volumes accordingly. In your IE, go to the following link: Aspirate fixative, rinse three times in 1X PBS for 5 min each.
For best results, allow mountant to cure overnight at room temperature. Immerse slides in dH 2 O.
Antigen Unmasking For Citrate: